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Biosystems 17 Ketosteroid Reagent

 

Biosystems 17 Ketosteroid Reagent

 

Biosystems 17 Ketosteroid Reagent STRUCTURE 1 x 5 ml. Aminoform of 0,7 mol/l, azid of sodium of 0,5 g/l. Harmful (Xn): R42/43: Causes irritation at inhalation and contact with skin. S18-24-28.1: To hold and open the container carefully. Protect skin from contact. At hit on skin immediately wash out a large amount of water.

Reagent. 1×500 ml. Potassium hydroxide of 1 mol/l in ethanol of 3,4 mol/l.

Caustically (C): R35: Can cause a burn. S26-36/37/39-45: in case of contact with eyes immediately wash out a large amount of water and ask for medical care. Use protective clothes, gloves, points/mask. At accident or an indisposition immediately ask for medical care.

Reagent. 1 x 250 ml anhydride of ethanol of 99% (analytical degree / ch.d.a).

Highly inflammable (F): R11: It is highly inflammable. S7: You hold the container densely closed. S16: You hold far from flame sources – not to smoke.

  1. Microcolumns. 2х20 Contain buffering neutralsmolu.11208 2
  2. Reagent. 2 x 20 ml. m-Dinitrobenzon, powder, 60 mmol/l in ethanol, after cultivation.
  3. Biosystems 17 Ketosteroid Reagent

Very toxically (T +), it is dangerous to environment (N): R26/27/28-33-50/53: Very toxically at inhalation, contact with skin and swallowing. It is dangerous to water organisms, can cause effect of accumulation in the water environment. S28.1-36/37-45-60-61: in case of contact with skin immediately wash out a large amount of water and ask for medical care. Use protective clothes, gloves, points/mask. At accident or an indisposition immediately ask for medical care. Material and/or the container has to be utilized as dangerous wastes. To avoid hit in environment. To follow special instructions / instructions of safety.

  1. Reagent. 1 x 90 ml. Hydroxide of potassium of 9 mol/l.Caustically (C): R22-35: It is dangerous when swallowing. Can cause a burn. S26-36/3739-45:in case of contact with eyes immediately wash out a large amount of water and ask for medical care. Use protective clothes, gloves, points/mask. At accident or an indisposition immediately ask for medical care.
  2. Reagent. 1 x 230 ml. Dichloromethane of 99%.

It is harmful (Xn): R40: The risk of irreversible effects is possible. Protect skin and eyes from contactUse protective clothes, gloves.

  1. Standard. 1 x 5 ml Degidroepiandrosteron (DGEA). 1000 mg/l = 3470 µmol/l, ethanol. Primary standard.

Highly inflammable (F): R11: It is highly inflammable. S7: You hold the container densely closed. S16: You hold far from flame sources – not to smoke

NECESSARY EQUIPMENT

Biosystems 17 Ketosteroid Reagent

− the Spectrophotometer or a photometer with the filter of 520 nanometers (515 – 525).

− Water thermobath

− the Boiling water bath

− Desktop centrifuge

SAMPLES

Biosystems 17 Ketosteroid Reagent

Urine. The daily urine collected on standard procedure.

To store at 2 – 8ºС and to use within 24 hours. Stability of a sample no more than 15 days at 2 – 8ºС or 1 month at-20ºС in case rn to finish them to 3-6 concentrated by hydrochloric acid (NSL). Before definition urine should be ottsentrifugirovat or filtered.

 

Biosystems 17 Ketosteroid Reagent

 

Brand: Biosystems