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Biosystems Acid Phostpase

04 Haziran 2017
2 kez görüntülendi
Biosystems Acid Phostpase

Biosystems Acid Phostpase

PRINCIPLE OF THE METHOD

Acid phosphatase (ACP) catalyzes in acid medium the hydrolysis of the phosphate group from
α-naphtyl phosphate.

The α-naphtol formed reacts with a diazonium salt (Fast Red TR)
originating a chromogen.

The catalytic concentration is determined from the rate of chromogenformation, measured at 405 nm1.

Pentanediol accelerates the reaction by acting as phosphate acceptor. Tartrate in used as a specific inhibitor of the prostatic fraction1,2

Biosystems Acid Phostpase

CONTENTS AND COMPOSITION
AT. Reagent. 1 x 45 mL. Sodium citrate 110 mmol/L, 1,5-pentanediol 220 mmol/L, pH 5.2.
AI. Reagent. 1 x 22.5 mL. Sodium citrate 110 mmol/L, 1,5-pentanediol 220 mmol/L, sodium
tartrate 110 mmol/L, pH 5.2.
B1. Reagent. 4 for 10 mL. α-Naphtyl phosphate 12.5 mmol/L, after dissolution.
B2. Reagent. 4 for 10 mL. Fast Red TR 1.25 mmol/L, after dissolution.
C. Reagent. 1 x 5 mL. Acetic acid 0.6 mol/L.
STORAGE
Store at 2-8ºC.
Reagents are stable until the expiry date shown on the label when stored tightly closed and if
contaminations are prevented during their use.
Indications of deterioration:
− Reagents: Presence of particulate material, turbidity, absorbance of the blank over 0.450 at
405 nm (1 cm cuvette).

Biosystems Acid Phostpase REAGENT PREPARATION

Working Reagent. Add 10 mL of Reagent AT (total ACP) or 10 mL of Reagent AI (non prostatic
ACP) to the Reagent B1.

Cap and shake until dissolved. Next, add this solution to the Reagent

B2. Cap and shake until dissolved. Stable for 10 days at 2-8ºC.
ADDITIONAL EQUIPMENT
− Analyzer, spectrophotometer or photometer with cell holder thermostatable at 30 or 37ºC and
able to read at 405 nm.
− Cuvettes with 1 cm light path.
SAMPLES
Serum collected by standard procedures. Acid phosphatase is unstable in serum. Measure
immediately or add 1 drop of Reagent C per mL serum.

Acid phosphatase in acidified serum is stable for 6 days at 2-8ºC. Hemolysed samples are not suitable for testing.

Biosystems Acid Phostpase PROCEDURE

Biosystems Acid Phostpase

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