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Biosystems Adenosine Deaminase

Biosystems Adenosine Deaminase

STORAGE Store at 2-8ºC.
Reagents are stable until the expiry date shown on the label when stored tightly closed and if contaminations are
prevented during their use. Indications of deterioration:
Reagents: Presence of particulate material, turbidity, absorbance of the blank lower the limit indicated in
“Assay parameters”.

 

AUXILIARY REAGENTS
S. ADA Standard 1 x 1 mL (BioSystems Cod. 18052). Bovine ADA, Tris 50 mmol/L. ADA concentration is given
on the vial label. ADA value is traceable to the reference material BCR-647 (IRMM).
Reconstitute with 1.0 mL of distilled water.Stable for 7 days at 2-8ºC or for 2 months at –18ºC when frozen
in aliquots.

REAGENT PREPARATION
Working Reagent: Add 2 mL of Reagent B into one bottle of Reagent A. Mix gently. Other volumes can be
prepared in the proportion: 4 mL Reagent A + 1 mL Reagent B. Stable for 30 days at 2-8ºC.
Reagent open and kept in the refrigerated compartment of the analyzer is stable 12 days.

ADDITIONAL EQUIPMENT
Analyzer, spectrophotometer or photometer with cell holder thermostatable at 37ºC and able to read at 340
nm.
SAMPLES

Pleural fluid or blood serum collected by standard procedures.
Adenosine deaminase in samples is stable for 7 days at 2-8ºC.

CALIBRATION
It is recommended to use an ADA Standard.
A calibration is recommended at least every 12 days, after reagent lot change or as required by quality control
procedure

 

Brand: Biosystems

 

Biosystems Adenosine Deaminase