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Biosystems Albumin Reagent

Biosystems Albumin Reagent

 

Biosystems Albumin Reagent for the measurement of albumin concentration in human serum or plasma. The obtained
values are useful as an aid in the evaluation of protein synthesis of the liver in the chronic liver
diseases and for the study of the nutritional status.
This reagent is for use in the BioSystems BA analyzers or in other analyzer with similar performance characteristics.
CLINICAL SIGNIFICANCE
Albumin is the most abundant protein in human plasma. It has three main functions: it contributes
towards maintaining the colloid oncotic pressure of plasma, it acts as non-specific transport vehicle for many nonpolar compounds and it is a source of endogenous amino acids.
Hyperalbuminemia is of little diagnostic significance except in dehydration1

Hypoalbuminemia is found as a result of several factors: reduced synthesis caused by liver
diseases; reduced absorption of amino acids due to malabsorption syndromes or malnutrition;
increased catabolism as a result of inflammation or tissue damage; altered distribution between
intravascular and extravascular space due to increased capillary permeability, overhydration or
ascites; abnormal losses caused by renal disease (nephrotic syndrome, diabetes mellitus, chronic
glomerulonephritis, systemic lupus erythematosus), gastrointestinal tract disease (ulcerative
colitis, Crohn’s disease) or skin damage (exfoliative dermatitis, extensive burns); congenital
absence of albumin or analbuminemia1,2

Albumin plasma concentrations, although important for management and follow-up, have very
little value in diagnosis1
.
Clinical diagnosis should not be made on the findings of a single test result, but should integrate
both clinical and laboratory data.
PRINCIPLE OF THE METHOD
Albumin in the sample reacts with bromocresol green in acid medium forming a coloured complex
that can be measured by spectrophotometry3
.

STRUCTURE

Biosystems Albumin Reagent

  1. Reagent. Acetate buffer of 100 mmol/l, bromokrezolovy green 0.27 mmol/l, cleaner, rn 4.1.
  2. Standard of Albumine. Bull albumine. Concentration is specified on a bottle label. The size of concentration corresponds to Recommendations for Standard materials 927 (National Institute of Standards and Technology, the USA).

NECESSARY EQUIPMENT

– The spectrophotometer or a photometer with the filter of 630 nanometers (610 – 670).

SAMPLES

Biosystems Albumin Reagent

Serum or plasma (with EDTA, citrate or heparin) collected on standard procedure.

Albumine in serum is stable within 3 days at 2-8ºC.

 

Brand: Biosystems