03423220016
Sosyal Medya Hesaplarımız

Biosystems Alpha Amylase Direct

Biosystems Alpha Amylase Direct

STRUCTURE

Biosystems Alpha Amylase Direct

A.Reagent. MES of 50 mmol/l, chloride of calcium of 5 mmol/l, chloride of sodium of 300 mmol/l, sodium thiocyanate of 450 mmol/l, CNP-G3 2.25 mmol/l, pH 6.1

A-amylase is stable in urine within 1 month at 2-8ºС if previously to finish rn to 7.

NECESSARY EQUIPMENT

– The analyzer, the spectrophotometer or a photometer with thermostatically controlled measuring kyuvety on 25, 30 or 37ºS with the filter of 405 nanometers

– Ditches with a length of optical way of 1 cm.

SAMPLES

The serum, plasma or urine received by means of standard procedures.

A-amylase is stable in serum or plasma within 1 month at 2-8ºС. Use heparin as anticoagulant.

INTENDED USE
Reagent for the measurement of -amylase concentration in human serum, plasma or urine.

Theobtained values are useful as an aid in the diagnosis and treatment of acute and chronic pancreatitis.

This reagent is for use in the BioSystems BA analyzers or in other analyzer with similar performance characteristics.

CLINICAL SIGNIFICANCE

Amylase catalyzes the hydrolysis of -1.4-linkages of carbohydrates constituted of

D-glucose units. The result is the formation of dextrans, maltose and some glucose molecules.

Amylase is produced mainly by the exocrine pancreas (P-type) and the salivary glands (S-type)but it is also found in other tissues.

Assays of amylase activity in serum and urine are largely of use in the diagnosis of pancreaticdiseases such as acute or chronic pancreatitis.

Hyperamylasemia can also be due to renal insufficiency, acute pain of the abdomen, tumors of the lungs and the ovaries, salivary glands

Clinical diagnosis should not be made on the findings of a single test result, but should integrate both clinical and laboratory data.

PRINCIPLE OF THE METHOD

Amylase catalyzes the hydrolysis of 2-chloro-4-nitrophenyl-malto-trioside (CNP-G3) to
2-chloro-4-nitrophenol (CNP).

The catalytic concentration is determined from the rate of 2-chloro4-nitrophenol formation, measured at 405 nm3-5

CNP – G3 CNP + maltotriose
CONTENTS
COD 21550 COD 23550
A. Reagent 8 x 20 mL 4 x 20 mL

COMPOSITION

A. Reagent: MES 50 mmol/L, calcium chloride 5 mmol/L, sodium chloride 300 mmol/L, sodium
thiocyanate 450 mmol/L, CNP-

STORAGE AND STABILITY Store at 2-8 ºC.

Components are stable once opened until the expiry date marked in the label if they are stored
well closed and care is taken to

On board stability: Reagents open and kept in the refrigerated compartment of the analyzer are
stable 2 months.

Indications of deterioration: Absorbance of the blank over the limit indicated in “Test Parameters”.

ADDITIONAL MATERIALS REQUIRED (NOT PROVIDED)

Biochemistry Calibrator (BioSystems cod. 18011) or Biochemistry Calibrator Human (BioSystems
cod. 18044).

REAGENT PREPARATION

Reagents are provided ready to use.