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Biosystems Alpha Amylase Direct

Alpha amlylase biyokimya kitleri

Biosystems Alpha Amylase Direct



Biosystems Alpha Amylase Direct

A.Reagent. MES of 50 mmol/l, chloride of calcium of 5 mmol/l, chloride of sodium of 300 mmol/l, sodium thiocyanate of 450 mmol/l, CNP-G3 2.25 mmol/l, pH 6.1

A-amylase is stable in urine within 1 month at 2-8ºС if previously to finish rn to 7.


– The analyzer, the spectrophotometer or a photometer with thermostatically controlled measuring kyuvety on 25, 30 or 37ºS with the filter of 405 nanometers

– Ditches with a length of optical way of 1 cm.


The serum, plasma or urine received by means of standard procedures.

A-amylase is stable in serum or plasma within 1 month at 2-8ºС. Use heparin as anticoagulant.

Reagent for the measurement of -amylase concentration in human serum, plasma or urine.

Theobtained values are useful as an aid in the diagnosis and treatment of acute and chronic pancreatitis.
This reagent is for use in the BioSystems BA analyzers or in other analyzer with similar performance characteristics.

Amylase catalyzes the hydrolysis of -1.4-linkages of carbohydrates constituted of
D-glucose units. The result is the formation of dextrans, maltose and some glucose molecules.
Amylase is produced mainly by the exocrine pancreas (P-type) and the salivary glands (S-type)but it is also found in other tissues.
Assays of amylase activity in serum and urine are largely of use in the diagnosis of pancreaticdiseases such as acute or chronic pancreatitis.

Hyperamylasemia can also be due to renal insufficiency, acute pain of the abdomen, tumors of the lungs and the ovaries, salivary glands
lesions, macroamylasemia, diabetic ketoacidosis, biliary tract disease, cerebral trauma, chronic alcoholism and drugs (opiates)1,2
Clinical diagnosis should not be made on the findings of a single test result, but should integrate both clinical and laboratory data.

Amylase catalyzes the hydrolysis of 2-chloro-4-nitrophenyl-malto-trioside (CNP-G3) to
2-chloro-4-nitrophenol (CNP).

The catalytic concentration is determined from the rate of 2-chloro4-nitrophenol formation, measured at 405 nm3-5

CNP – G3 CNP + maltotriose
COD 21550 COD 23550
A. Reagent 8 x 20 mL 4 x 20 mL

A. Reagent: MES 50 mmol/L, calcium chloride 5 mmol/L, sodium chloride 300 mmol/L, sodium
thiocyanate 450 mmol/L, CNP-G3 2.25 mmol/L, pH 6.1.
Store at 2-8 ºC.
Components are stable once opened until the expiry date marked in the label if they are stored
well closed and care is taken to prevent contamination during their use.
On board stability: Reagents open and kept in the refrigerated compartment of the analyzer are
stable 2 months.
Indications of deterioration: Absorbance of the blank over the limit indicated in “Test Parameters”.
Biochemistry Calibrator (BioSystems cod. 18011) or Biochemistry Calibrator Human (BioSystems
cod. 18044).
Reagents are provided ready to use.
Serum, plasma or urine collected by standard procedures.
-Amylase in serum or plasma is stable for 30 days at 2-8ºC. Use heparin as anticoagulant.
-Amylase in urine is stable for 30 days at 2-8ºC if pH is adjusted to approximately 7 before
storage. Centrifuge or filter before testing.
A reagent blank should be done every day and a calibration at least every 2 months, after reagent
lot change or as required by quality control procedures.
It is recommended to use the Biochemistry Control Serum level I (cod. 18005, cod. 18009 and
cod. 18042) and II (cod. 18007, cod. 18010 and cod. 18043) and the Biochemistry Control Urine
(cod. 18054 and cod. 18066) to verify the accuracy of the measurement procedure.
Each laboratory should establish its own internal Quality Control sch