Biosystems Aspartate Aminotransferase (AST-GOT)
PRINCIPLE OF THE METHOD: Aspartate aminotransferase (AST or GOT) catalyzes the transfer of the amino group from
aspartate to 2-oxoglutarate, forming oxalacetate and glutamate.
The catalytic concentration isdetermined from the rate of decrease of NADH, measured at 340 nm, by means of the malate
dehydrogenase (MDH) coupled reaction1,2,3
Aspartate + 2 – Oxoglutarate Oxalacetate + Glutamate
Oxalacetate + NADH + H+
Malate + NAD+
WARNING: H302: Harmful if swallowed. EUH031: Contact with acids liberates toxic gas.
P301+P312: IF SWALLOWED:
Call a POISON CENTER or doctor/physician if you feel unwell. P330: Rinse mouth.
For further warnings and precautions, see the product safety data sheet (SDS).
Biosystems Aspartate Aminotransferase (AST-GOT) STORAGE
Store at 2-8ºC.
Reagents are stable until the expiry date shown on the label when stored tightly closed and if
contaminations are prevented during their use.
Indications of deterioration:
Reagents: Presence of particulate material, turbidity, absorbance of the blank lower than 1.400
at 340 nm (1 cm cuvette).
C. Reagent (cod 11666): Pyridoxal phosphate 10 mmol/L. 5 mL.
Biosystems Aspartate Aminotransferase (AST-GOT) REAGENT PREPARATION
Working Reagent: Pour the contents of the Reagent B into the Reagent A bottle. Mix gently. Other
volumes can be prepared in the proportion:
4 mL Reagent A + 1 mL Reagent B (Note 1). Stablefor 1 month at 2-8ºC.
Analyzer, spectrophotometer or photometer with cell holder thermostatable at 30 or 37ºC and
able to read at 340 nm.
Cuvettes with 1 cm light path.
Serum and plasma collected by standard procedures.
Aspartate aminotransferase in serum and plasma is stable for 7 days at 2-8ºC. Use heparin as
Firmamız Müşterilerimizin isteği doğrultusunda tamamı güvenilir ve stabil olmak kaydıyla, yaygın kullanım alanına sahip Biosystems reaktif Ve Cihazlarının satışını yapmaktayız.