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Metanephrines Biosystems Reagent

 

Metanephrines Biosystems Reagent

 

Metanephrines Biosystems Reagent PRINCIPLE OF THE METHOD: Metanephrines are retained by a cationic exchange resin, being eluted thereafter once the interfering substances are washed away.

The metanephrines are quantified spectrophotometrically as vanillin after
periodate oxidation under alkaline conditions.

CONTENTS AND COMPOSITION
1. Reagent. 1 x 225 mL. Imidazole buffer 0.4 mol/L, sodium EDTA 2.7 mmol/L, preservative.
2. Reagent. 1 x 400 mL. Ammonia 4 mol/L.

WARNING: H315: Causes skin irritation. H319: Causes serious eye irritation. H335: May cause respiratory
irritation. P280: Wear protective gloves/protective clothing/eye protection/face protection. P304+P340: IF

DANGER: H302: Harmful if swallowed. H318: Causes serious eye damage. EUH031: Contact with acids
liberates toxic gas.
S. Standard. 2 x 5 mL. Normetanephrine 100 mg/L = 550 µmol/L in hydrochloric acid 0.1 mol/L. Aqueous
primary standard.
For further warnings and precautions, see the product safety data sheet (SDS).

Metanephrines Biosystems Reagent

STORAGE
Store at 2-30 ºC.
Reagents and Standard are stable until the expiry date shown on the label when stored tightly closed and if
contamination is prevented during their use.
Indications of deterioration:
− Reagents: Presence of particulate material, turbidity, absorbance of the reagent blank over 0.060 at 360 nm
(1 cm cuvette).
− Microcolumns: Absence of buffer over the upper filter.

ADDITIONAL REAGENTS

− Concentrated hydrochloric acid (analytical grade).

REAGENT PREPARATION
Reagents (B) and (C): Dissolve the dry powder in 10 mL of distilled water. Stable for 5 months at 2-8ºC.

ADDITIONAL EQUIPMENT
− Boiling water bath
− Spectrophotometer with a 360 nm filter (358-362)

SAMPLES
Urine. 24 hours specimen collected by standard procedures.
Boric acid is not an acceptable preservative.
Keep at 2-8ºC and use within 24 hours. Samples can be stored at 2-8ºC for a maximum of 15 days at 2-8ºC or 

Metanephrines Biosystems Reagent

 

 

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Brand Name
Biosystems
Product Name
Metanephrines Biosystems Reagent
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