Biosystems Albumine Reagent
- INTENDED USE
- Reagent for the measurement of albumin concentration in human serum or plasma.
- The obtainedvalues are useful as an aid in the evaluation of protein synthesis of the liver in the chronic liver
diseases and for the study of the nutritional status.
- This reagent is for use in the BioSystems BA analyzers or in other analyzer with similar
- Albumin is the most abundant protein in human plasma.
- It has three main functions: it contributes towards maintaining the colloid oncotic pressure of plasma, it acts as non-specific transport
vehicle for many nonpolar compounds and it is a source of endogenous amino acids.
- Hyperalbuminemia is of little diagnostic significance except in dehydration1
- Hypoalbuminemia is found as a result of several factors: reduced synthesis caused by liver
diseases; reduced absorption of amino acids due to malabsorption syndromes or malnutrition;
increased catabolism as a result of inflammation or tissue damage;
- Altered distribution between intravascular and extravascular space due to increased capillary permeability, overhydration or
ascites; abnormal losses caused by renal disease (nephrotic syndrome, diabetes mellitus, chronic
glomerulonephritis, systemic lupus erythematosus), gastrointestinal tract disease (ulcerative
colitis, Crohn’s disease) or skin damage (exfoliative dermatitis, extensive burns); congenital
absence of albumin or analbuminemia1,2
- Albumin plasma concentrations, although important for management and follow-up, have very
little value in diagnosis1
- Clinical diagnosis should not be made on the findings of a single test result, but should integrate
both clinical and laboratory data.
PRINCIPLE OF THE METHOD
- Albumin in the sample reacts with bromocresol green in acid medium forming a coloured complex
that can be measured by spectrophotometry3
- A. Reagent. Acetate buffer 100 mmol/L, bromocresol green 0.27 mmol/L, detergent, pH 4.1.
STORAGE AND STABILITY
- Store at 2-8 ºC.
Components are stable once opened until the expiry date marked in the label if they are stored
well closed and care is taken to prevent contamination during their use.
- On board stability: Reagents open and kept in the refrigerated compartment of the analyzer are
stable 2 months.
- Indications of deterioration: Absorbance of the blank over the limit indicated in “Test Parameters”.
ADDITIONAL MATERIALS REQUIRED (NOT PROVIDED)
- Biochemistry Calibrator (BioSystems cod. 18011) or Biochemistry Calibrator Human (BioSystems
- Reagents are provided ready to use.
- Serum or plasma (EDTA, citrate or heparine) collected by standard procedures.
- Albumin in serum is stable for 3 days at 2-8ºC.
- A reagent blank should be done every day and a calibration at least every 2 months, after reagent
lot change or as required by quality control procedure
- It is recommended to use the Biochemistry Control Serum level I (cod. 18005, 18009 and 18042)
and II (cod. 18007, 18010 and 18043) to verify the accuracy of the measurement procedure.
- Each laboratory should establish its own internal Quality Control scheme and procedures for
corrective action if control results are not within the acceptable limits.