Biosystems Albumine Reagent

Albumin in the sample reacts with bromocresol green in acid medium forming a coloured complex
that can be measured by BTS 350 spectrophotometry

Biosystems Albumine Reagent INTENDED USE

Reagent for the measurement of albumin concentration in human serum or plasma.

The obtainedvalues are useful as an aid in the evaluation of protein synthesis of the liver in the chronic liver
diseases and for the study of the nutritional status.

This reagent is for use in the BioSystems BA analyzers or in other analyzer with similar
performance characteristics.


Albumin is the most abundant protein in human plasma.

It has three main functions: it contributes towards maintaining the colloid oncotic pressure of plasma, it acts as non-specific transport
vehicle for many nonpolar compounds and it is a source of endogenous amino acids.

Hyperalbuminemia is of little diagnostic significance except in dehydration1

Hypoalbuminemia is found as a result of several factors: reduced synthesis caused by liver
diseases; reduced absorption of amino acids due to malabsorption syndromes or malnutrition;
increased catabolism as a result of inflammation or tissue damage;

Altered distribution between intravascular and extravascular space due to increased capillary permeability, overhydration or.

Ascites; abnormal losses caused by renal disease (nephrotic syndrome, diabetes mellitus, chronic
glomerulonephritis, systemic lupus erythematosus), gastrointestinal tract disease (ulcerative
colitis, Crohn’s disease) or .

Skin damage (exfoliative dermatitis, extensive burns); congenital absence of albumin or analbuminemia1,2

Albumin plasma concentrations, although important for management and follow-up, have very
little value in diagnosis1

Clinical diagnosis should not be made on the findings of a single test result, but should integrate
both clinical and laboratory data.


Albumin in the sample reacts with bromocresol green in acid medium forming a coloured complex
that can be measured by spectrophotometry3


  1. Reagent. Acetate buffer 100 mmol/L, bromocresol green 0.27 mmol/L, detergent, pH 4.1.

Biosystems Albumine Reagent STORAGE AND STABILITY

Store at 2-8 ºC.
Components are stable once opened until the expiry date marked in the label if they are stored
well closed and care is taken to prevent contamination during their use.

On board stability: Reagents open and kept in the refrigerated compartment of the analyzer are
stable 2 months.

Indications of deterioration: Absorbance of the blank over the limit indicated in “Test Parameters”.


Biochemistry Calibrator (BioSystems cod. 18011) or Biochemistry Calibrator Human (BioSystems
cod. 18044).


Reagents are provided ready to use.


Serum or plasma (EDTA, citrate or heparine) collected by standard procedures.

Albumin in serum is stable for 3 days at 2-8ºC.


A reagent blank should be done every day and a calibration at least every 2 months, after reagent
lot change or as required by quality control procedure

Biosystems Albumine Reagent QUALITY CONTROL

It is recommended to use the Biochemistry Control Serum level I (cod. 18005, 18009 and 18042)
and II (cod. 18007, 18010 and 18043) to verify the accuracy of the measurement procedure.

Each laboratory should establish its own internal Quality Control scheme and procedures for
corrective action if control results are not within the acceptable limits.




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