Biosystems Reagent Vanilmindalnaya Acid PRINCIPLE OF THE METHOD
Vanilmandelic acid (VMA) is retained by an anionic exchange resin, being eluted thereafter once the interfering
substances are washed away. The VMA is quantified spectrophotometrically as vanillin after periodate oxidation
under alkaline conditions1,2,3
Store at 2-30ºC.
Reagents and Standard are stable until the expiry date shown on the label when stored tightly closed and if
contamination is prevented during their use.
Indications of deterioration:
− Reagents: Presence of particulate material, turbidity, absorbance of the reagent blank over 0.050 at 360 nm.
− Microcolumns: Absence of buffer over the resin bed.
− Concentrated hydrochloric acid (analytical grade).
Reagents (B) and (C): Dissolve the dry powder in 10 mL of distilled water. Stable for 5 months at 2-8ºC.
Standard (S): Dissolve the dry powder in 5 mL of Reagent (3) and add one drop of concentrated hydrochloric
acid. Stable for 2 months at 2-8ºC.
− Spectrophotometer or photometer with a 360 nm filter (358-362).
− Thermostatic water bath.
Biosystems Reagent Vanilmindalnaya Acid SAMPLES
Urine. 24 hours specimens collected by standard procedures.
Keep at 2-8ºC and use within 24 hours. Samples can be stored at 2-8ºC for a maximum of 5 days or 1 month at
−20ºC if adjusted to pH 1-2 with concentrated hydrochloric acid (HCl). Centrifuge or filter before testing
- Iron Binding Capacity
- 11554TOTAL IRON BINDING CAPACITY
- 11835UNSATURATED IRON BINDING CAPACITY (UIBC)
- 11583alpha-AMYLASE – DIRECT
- 11534alpha-AMYLASE – EPS