Biosystems Reagent of AST/GOT INTENDED USE
Reagent for the measurement of aspartate aminotransferase (AST or GOT) concentration inhuman serum or plasma.
The obtained values are useful as an aid in the diagnosis andmonitoring of disorders of liver diseases, especially acute ones.
This reagent is for use in the BioSystems BA analyzers or in other analyzer with similarperformance characteristics.
The aminotransferases catalyze the formation of glutamic acid from 2-oxoglutarate by transfer of amino groups.
AST is found in highest concentration in the liver and heart muscle but it is also abundant in skeletal muscle, kidney and pancreas.
The serum concentration of AST is elevated in hepatitis and other forms of.
Hepatic diseaseassociated with necrosis: infectious mononucleosis, cholestasis, cirrhosis, metastasic carcinoma
Serum AST concentration is also elevated after myocardial infarction, in sketetal muscle disease(as progressive muscular distrophy), in acute pancreatitis or hemolytic disease and other1,2
Clinical diagnosis should not be made on the findings of a single test result, but should integrate both clinical and laboratory data.
Biosystems Reagent of AST/GOT PRINCIPLE OF THE METHOD
Aspartate aminotransferase (AST or GOT) catalyzes the transfer of the amino group fromaspartate to 2-oxoglutarate, forming oxalacetate and glutamate.
The catalytic concentration isdetermined from the rate of decrease of NADH, measured at 340 nm, by means of the malate dehydrogenase (MDH) coupled reaction3-6