Sesmed Medikal

Biosystems Biyokimya Kitleri

Biosystems Albumine Reagent

Biosystems Albumine Reagent


  • Reagent for the measurement of albumin concentration in human serum or plasma.
  • The obtainedvalues are useful as an aid in the evaluation of protein synthesis of the liver in the chronic liver
    diseases and for the study of the nutritional status.
  • This reagent is for use in the BioSystems BA analyzers or in other analyzer with similar
    performance characteristics.
  • Albumin is the most abundant protein in human plasma.
  • It has three main functions: it contributes towards maintaining the colloid oncotic pressure of plasma, it acts as non-specific transport
    vehicle for many nonpolar compounds and it is a source of endogenous amino acids.
  • Hyperalbuminemia is of little diagnostic significance except in dehydration1
  • Hypoalbuminemia is found as a result of several factors: reduced synthesis caused by liver
    diseases; reduced absorption of amino acids due to malabsorption syndromes or malnutrition;
    increased catabolism as a result of inflammation or tissue damage;
  • Altered distribution between intravascular and extravascular space due to increased capillary permeability, overhydration or
    ascites; abnormal losses caused by renal disease (nephrotic syndrome, diabetes mellitus, chronic
    glomerulonephritis, systemic lupus erythematosus), gastrointestinal tract disease (ulcerative
    colitis, Crohn’s disease) or skin damage (exfoliative dermatitis, extensive burns); congenital
    absence of albumin or analbuminemia1,2
  • Albumin plasma concentrations, although important for management and follow-up, have very
    little value in diagnosis1
  • Clinical diagnosis should not be made on the findings of a single test result, but should integrate
    both clinical and laboratory data.


  • Albumin in the sample reacts with bromocresol green in acid medium forming a coloured complex
    that can be measured by spectrophotometry3


  • A. Reagent. Acetate buffer 100 mmol/L, bromocresol green 0.27 mmol/L, detergent, pH 4.1.


  • Store at 2-8 ºC.
    Components are stable once opened until the expiry date marked in the label if they are stored
    well closed and care is taken to prevent contamination during their use.
  • On board stability: Reagents open and kept in the refrigerated compartment of the analyzer are
    stable 2 months.
  • Indications of deterioration: Absorbance of the blank over the limit indicated in “Test Parameters”.


  • Biochemistry Calibrator (BioSystems cod. 18011) or Biochemistry Calibrator Human (BioSystems
    cod. 18044).


  • Reagents are provided ready to use.


  • Serum or plasma (EDTA, citrate or heparine) collected by standard procedures.
  • Albumin in serum is stable for 3 days at 2-8ºC.


  • A reagent blank should be done every day and a calibration at least every 2 months, after reagent
    lot change or as required by quality control procedure


  • It is recommended to use the Biochemistry Control Serum level I (cod. 18005, 18009 and 18042)
    and II (cod. 18007, 18010 and 18043) to verify the accuracy of the measurement procedure.
  • Each laboratory should establish its own internal Quality Control scheme and procedures for
    corrective action if control results are not within the acceptable limits.