Sesmed Medikal

Biosystems Biyokimya Kitleri

Biosystems Fibrinogen Reagent

Reagents are stable until the expiry date shown on the label when stored tightly closed

Biosystems Fibrinogen Reagent

PRINCIPLE OF THE METHOD
Fibrinogen in the sample precipitates in the presence of anti-human fibrinogen antibodies. The
light scattering of the antigen-antibody complexes is proportional to the fibrinogen concentration
and can be measured by turbidimetry1

CONTENTS AND COMPOSITION
A. Reagent: 1 x 40 mL. Phosphate buffer, sodium azide 0.95 g/L, pH 7.5.
B. Reagent: 1 x 10 mL. Goat anti-human fibrinogen antibodies, sodium azide 0.95 g/L.
STORAGE
Store at 2-8ºC.
Reagents are stable until the expiry date shown on the label when stored tightly closed and if
contaminations are prevented during their use.
Indications of deterioration: Presence of particulate material, turbidity, absorbance of the blank
over 0.300 at 340 nm.
AUXILIARY REAGENTS
S. Fibrinogen Standard (BioSystems cod. 31601). Concentration is given on the vial label. The
concentration value is traceable to the WHO 2nd International Standard for Fibrinogen plasma,
98/612 (National Institute for Biological Standards and Control, NIBSC).
Human plasma used in the preparation of the standard has been tested and found to be
negative for the presence of antibodies anti-HIV and anti-HCV, as well as for HBs antigen.
However, the standard should be handled cautiously as potentially infectious.
Reconstitute with 1 mL of distilled water. Stable for 2 days at 2-8ºC.
Calibration curve: Prepare dilutions of the standard using 9 g/L saline as diluent. Multiply the
concentration of the fibrinogen standard by the corresponding factor indicated below to obtain
the fibrinogen concentration of the dilutions.

REAGENT PREPARATION
Reagents are provided ready to use.
ADDITIONAL EQUIPMENT
− Thermostatic water bath at 37ºC.
− Analyzer, spectrophotometer or photometer with cell holder thermostatable at 37ºC and able
to read at 340 ± 20 nm.
SAMPLES
Plasma collected by standard procedures. Use sodium citrate as anticoagulant.
Plasma fibrinogen is stable for 2 days at 2-8°C.

 

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