Sesmed Medikal

Biosystems Biyokimya Kitleri

Biosystems immunoglobulin M reagent

Biosystems immunoglobulin M reagent is provided ready to use.

Biosystems immunoglobulin M reagent

PRINCIPLE OF THE METHOD
Immunoglobulin M in the sample precipitates in the presence of anti-human immunoglobulin M
antibodies. The light scattering of the antigen-antibody complexes is proportional to the
immunoglobulin M concentration and can be measured by turbidimetry1,2

STORAGE
Store at 2-8ºC.
The Reagent is stable until the expiry date shown on the label when stored tightly closed and if
contaminations are prevented during its use.
Indications of deterioration: Presence of particulate material, turbidity, absorbance of the blank
over 0.200 at 340 nm.
ADDITIONAL REAGENTS
− Protein Calibrators (BioSystems cod. 31075). The set contains 5 different levels of IgM
concentration and it should be used to prepare the calibration curve. The calibrators are
supplied ready to use.
REAGENT PREPARATION
Reagent is provided ready to use.
ADDITIONAL EQUIPMENT
− Thermostatic water bath at 37ºC.
− Analyzer, spectrophotometer or photometer with cell holder thermostatable at 37ºC and able
to read at 340 ± 20 nm.
SAMPLES
Serum or plasma collected by standard procedures. Use heparin or EDTA as anticoagulants.
Lipemic samples are not suitable for testing.
Serum or plasma IgM is stable for 7 days at 2-8°C.

Biosystems immunoglobulin M reagent

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