Sesmed Medikal

Biosystems Prealbumin Reagent

The Reagent is stable until the expiry date shown on the label when stored tightly closed and if
contaminations are prevented during its use

Biosystems Prealbumin Reagent

PRINCIPLE OF THE METHOD
Prealbumin, also known as transthyretin, in the sample precipitates in the presence of antihuman prealbumin antibodies. The light scattering of the antigen-antibody complexes is
proportional to the prealbumin concentration and can be measured by turbidimetry1,2
COMPOSITION
A. Reagent 2 x 60 mL: Imidazole buffer 0.1 mol/L, goat anti-human PAB antibodies, sodium
azide 0.95 g/L, pH 7.5.
STORAGE
Store at 2-8ºC.
The Reagent is stable until the expiry date shown on the label when stored tightly closed and if
contaminations are prevented during its use.
Indications of deterioration: Presence of particulate material, turbidity, absorbance of the blank
over 0.300 at 340 nm.
AUXILIARY REAGENTS
− Protein Calibrators (BioSystems Cod. 31075). The set contains 5 different levels of
prealbumin concentration and it should be used to prepare the calibration curve. The
calibrators are supplied ready to use.
SAMPLES
Serum or plasma collected by standard procedures (use heparin or EDTA as anticoagulants).
Serum or plasma prealbumin is stable for 6 months at 2-8°C.3

REFERENCE VALUES
Serum, adults4
: 20 – 40 mg/dL = 0.20 – 0.40 g/L.
This range is given for orientation only; each laboratory should establish its own reference
range.
CALIBRATION
It is recommended to use a serum based calibrator (Protein Calibrator, cod. 31075).
A calibration is recommended at least every 2 months, after reagent lot change or as required by
quality control procedures.
ASSAY PARAMETERS
These reagents may be used in several automatic analysers. Specific instructions for application
in many of them are available on request.
− Wavelength: 340 nm
− Temperature: 37ºC
− Analysis mode: end point

Biosystems Prealbumin Reagent