Biosystems Reagent of (AST/GOT)
Reagent for the measurement of aspartate aminotransferase (AST or GOT) concentration in
human serum or plasma. The obtained values are useful as an aid in the diagnosis and
monitoring of disorders of liver diseases, especially acute ones.
This reagent is for use in the BioSystems BA analyzers or in other analyzer with similar
The aminotransferases catalyze the formation of glutamic acid from 2-oxoglutarate by transfer of
amino groups. AST is found in highest concentration in the liver and heart muscle but it is also
abundant in skeletal muscle, kidney and pancreas.
The serum concentration of AST is elevated in hepatitis and other forms of hepatic disease
associated with necrosis: infectious mononucleosis, cholestasis, cirrhosis, metastasic carcinoma
of the liver, delirium tremens, and after administration of various drugs1,2
Serum AST concentration is also elevated after myocardial infarction, in sketetal muscle disease
(as progressive muscular distrophy), in acute pancreatitis or hemolytic disease and other1,2
Clinical diagnosis should not be made on the findings of a single test result, but should integrate
both clinical and laboratory data.
PRINCIPLE OF THE METHOD
Aspartate aminotransferase (AST or GOT) catalyzes the transfer of the amino group from
aspartate to 2-oxoglutarate, forming oxalacetate and glutamate. The catalytic concentration is
determined from the rate of decrease of NADH, measured at 340 nm, by means of the malate
dehydrogenase (MDH) coupled reaction3-6