Biosystems Reagent Vanilmindalnaya Acid
PRINCIPLE OF THE METHOD
Vanilmandelic acid (VMA) is retained by an anionic exchange resin, being eluted thereafter once the interfering
substances are washed away. The VMA is quantified spectrophotometrically as vanillin after periodate oxidation
under alkaline conditions1,2,3
CONTENTS AND COMPOSITION
1. Reagent. 1×25 mL. Sodium phosphate buffer 0.6 mol/L pH 7.0, sodium azide 15 mmol/L.
2. Reagent. 1×120 mL. Sodium acetate buffer 0.2 mol/L pH 6.1, sodium azide 15 mmol/L.
3. Reagent. 1×170 mL. Sodium chloride 2 mol/L, sodium azide 15 mmol/L.
4. Microcolumns. 1×20. Contain a pre-weighted amount of a buffered anionic exchange resin and sodium azide
A. Reagent. 1×30 mL. Potassium carbonate 3 mol/L.
WARNING: H302: Harmful if swallowed. H315: Causes skin irritation. H319: Causes serious eye irritation.
H335: May cause respiratory irritation. P301+P312: IF SWALLOWED: Call a POISON CENTER or
doctor/physician if you feel unwell. P302+P352: IF ON SKIN: Wash with plenty of soap and water.
P304+P340: IF INHALED: Remove victim to fresh air and keep at rest in a position comfortable for
breathing. P305+P351+P338: IF IN EYES: Rinse cautiously with water for several minutes. Remove contact
lenses, if present and easy to do. Continue rinsing. P333+P313: If skin irritation or rash occurs: Get medical
B. Reagent. 1 for 10 mL. Sodium m-periodate powder 0.12 mol/L, after reconstitution.
DANGER: H272: May intensify fire; oxidiser. H301: Toxic if swallowed. H315: Causes skin irritation. H319:
Causes serious eye irritation. H335: May cause respiratory irritation. P301+P310: IF SWALLOWED:
Immediately call a POISON CENTER or doctor/physician. P305+P351+P338: IF IN EYES: Rinse cautiously
with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
C. Reagent. 1 for 10 mL. Sodium metabisulfite powder 0.67 mol/L, after reconstitution.
DANGER: H302: Harmful if swallowed. H318: Causes serious eye damage. EUH031: Contact with acids
liberates toxic gas.
S. Standard. 1 for 5 mL. Concentration of reconstituted standard is given on the label. The concentration value
is traceable to the Standard Reference Material 925 (National Institute of Standards and Technology, USA).
For further warnings and precautions, see the product safety data sheet (SDS).
Store at 2-30ºC.
Reagents and Standard are stable until the expiry date shown on the label when stored tightly closed and if
contamination is prevented during their use.
Indications of deterioration:
− Reagents: Presence of particulate material, turbidity, absorbance of the reagent blank over 0.050 at 360 nm.
− Microcolumns: Absence of buffer over the resin bed.
− Concentrated hydrochloric acid (analytical grade).
Reagents (B) and (C): Dissolve the dry powder in 10 mL of distilled water. Stable for 5 months at 2-8ºC.
Standard (S): Dissolve the dry powder in 5 mL of Reagent (3) and add one drop of concentrated hydrochloric
acid. Stable for 2 months at 2-8ºC.
− Spectrophotometer or photometer with a 360 nm filter (358-362).
− Thermostatic water bath.
Urine. 24 hours specimens collected by standard procedures.
Keep at 2-8ºC and use within 24 hours. Samples can be stored at 2-8ºC for a maximum of 5 days or 1 month at
−20ºC if adjusted to pH 1-2 with concentrated hydrochloric acid (HCl). Centrifuge or filter before testing