Biosystems Cholesterol Reagent
- A. Reagent. Pipes 35 mmol/L, sodium cholate 0.5 mmol/L, phenol 28 mmol/L, cholesterol esterase 0.2 U/mL, cholesterol oxidase 0.1 U/mL, peroxidase 0.8 U/mL,
4-aminoantipyrine 0.5 mmol/L, pH 7.0.S. Cholesterol Standard. Cholesterol 200 mg/dL (5,18 mmol/L). Aqueous primary standard.
- Store at 2-8ºC.
- Reagent and Standard are stable until the expiry date shown on the label when stored tightly closed and if contaminations are prevented during their use.
- Indications of deterioration:Reagent: Presence of particulate material, turbidity, absorbance of the blank over 0.200 at 500 nm (1 cm cuvette).
- Standard: Presence of particulate material, turbidity.
- Reagent and Standard are provided ready to use.
- Thermostatic water bath at 37ºC
- Analyzer, spectrophotometer or photometer able to read at 500 20 nm
- Serum or plasma collected by standard procedures.
- Cholesterol is stable for 7 days at 2-8ºC. Heparin, EDTA, oxalate and fluoride may be used as anticoagulant
- Cholesterol is a steroid of high molecular weight and possesses the cyclopentanophenanthrene skeleton.
- Dietary cholesterol is partially absorbed and it is also synthesized by the liver andother tissues.
- Cholesterol is transported in plasma by lipoproteins. It is excreted unchanged into bile or after transformation to bile acids.
- Increased total cholesterol values are associated with a progressively escalating risk of atherosclerosis and coronary artery disease5,6
- Clinical diagnosis should not be made on the findings of a single test result, but should integrate both clinical and laboratory data.