Biosystems Alaninaminotransferaz (ALT/GPT)
PRINCIPLE OF THE METHOD
Alanine aminotransferase (ALT or GPT) catalyzes the transfer of the amino group from alanine
to 2-oxoglutarate, forming pyruvate and glutamate. The catalytic concentration is determined
from the rate of decrease of NADH, me
A. Reagent: Tris 150 mmol/L, L-alanine 750 mmol/L, lactate dehydrogenase > 1350 U/L, pH
B. Reagent: NADH 1.9 mmol/L, 2-oxoglutarate 75 mmol/L, Sodium hydroxide 148 mmol/L,
sodium azide 9.5 g/L.
WARNING: H302: Harmful if swallowed. EUH031: Contact with acids liberates toxic gas.
P301+P312: IF SWALLOWED: Call a POISON CENTER or doctor/physician if you feel
unwell. P330: Rinse mouth.
For further warnings and precautions, see the product safety data sheet (SDS).
Store at 2-8ºC.
Reagents are stable until the expiry date shown on the label when stored tightly closed and if
contaminations are prevented during their use.
Indications of deterioration:
Reagents: Presence of particulate material, turbidity, absorbance of the blank lower than
1.400 at 340 nm (1 cm cuvette).
C. Reagent (cod 11666): Pyridoxal phosphate 10 mmol/L. 5 mL.
Working Reagent: Pour the contents of the Reagent B into the Reagent A bottle. Mix gently. Other volumes can
be prepared in the proportion: 4 mL Reagent A + 1 mL Reagent B (Note 1). Stable for 1 month at 2-8ºC.
Working Reagent with Pyridoxal Phosphate (Note 2): Mix as follows: 10 mL of Working Reagent + 0.1 mL of
Reagent C (cod 11666). Stable for 6 days at 2-8ºC.
Analyzer, spectrophotometer or photometer with cell holder thermostatable at 30 or 37ºC and
able to read at 340 nm.
Cuvettes with 1 cm light path.
Serum and plasma collected by standard procedures.
Alanine aminotransferase in serum and plasma is stable for 7 days at 2-8ºC. Use heparin or
EDTA as anticoagulant7