Biosystems Pancreatic Alpha Amylase
- Reagent for the measurement of -amylase concentration in human serum, plasma or urine.
- Theobtained values are useful as an aid in the diagnosis and treatment of acute and chronic pancreatitis.
- This reagent is for use in the BioSystems BA analyzers or in other analyzer with similar performance characteristics.
- Amylase catalyzes the hydrolysis of -1.4-linkages of carbohydrates constituted of
- D-glucose units. The result is the formation of dextrans, maltose and some glucose molecules.
- Amylase is produced mainly by the exocrine pancreas (P-type) and the salivary glands (S-type)but it is also found in other tissues.
- Assays of amylase activity in serum and urine are largely of use in the diagnosis of pancreatic diseases such as acute or chronic pancreatitis.
- Hyperamylasemia can also be due to renal insufficiency, acute pain of the abdomen, tumors of the lungs and the ovaries, salivary glands
lesions, macroamylasemia, diabetic ketoacidosis, biliary tract disease, cerebral trauma, chronic alcoholism and drugs (opiates)1,2
- Clinical diagnosis should not be made on the findings of a single test result, but should integrate both clinical and laboratory data.
PRINCIPLE OF THE METHOD
- Amylase catalyzes the hydrolysis of 2-chloro-4-nitrophenyl-malto-trioside (CNP-G3) to 2-chloro-4-nitrophenol (CNP). The catalytic concentration is determined from the rate of 2-chloro4-nitrophenol formation, measured at 405 nm3-5