Biosystems Fibrinogen Reagent

Bts 350 semi automatic Analyzer, spectrophotometer or photometer with cell holder thermostatable at 37┬║C and ableto read at 340 ┬▒ 20 nm.

Biosystems Fibrinogen Reagent PRINCIPLE OF THE METHOD

Fibrinogen in the sample precipitates in the presence of anti-human fibrinogen antibodies. The
light scattering of the antigen-antibody complexes is proportional to the fibrinogen concentration
and can be measured by turbidimetry1

CONTENTS AND COMPOSITION

A. Reagent: 1 x 40 mL. Phosphate buffer, sodium azide 0.95 g/L, pH 7.5.
B. Reagent: 1 x 10 mL. Goat anti-human fibrinogen antibodies, sodium azide 0.95 g/L.

STORAGE

Store at 2-8┬║C.
Reagents are stable until the expiry date shown on the label when stored tightly closed and if
contaminations are prevented during their use.

Indications of deterioration: Presence of particulate material, turbidity, absorbance of the blank
over 0.300 at 340 nm.

AUXILIARY REAGENTS

S. Fibrinogen Standard (BioSystems cod. 31601). Concentration is given on the vial label. The
concentration value is traceable to the.

WHO 2nd International Standard for Fibrinogen plasma.98/612 (National Institute for Biological Standards and Control, NIBSC).

Human plasma used in the preparation of the standard has been tested and found to be
negative for the presence of antibodies anti-HIV and anti-HCV, as well as for .

HBs antigen.However, the standard should be handled cautiously as potentially infectious.

Reconstitute with 1 mL of distilled water. Stable for 2 days at 2-8┬║C.

Calibration curve: Prepare dilutions of the standard using 9 g/L saline as diluent. Multiply the
concentration of the fibrinogen standard by the corresponding factor indicated below to obtain
the fibrinogen concentration of the dilutions.

REAGENT PREPARATION

Reagents are provided ready to use.

Biosystems Fibrinogen Reagent ADDITIONAL EQUIPMENT

Ôłĺ Thermostatic water bath at 37┬║C.
Ôłĺ Analyzer, spectrophotometer or photometer with cell holder thermostatable at 37┬║C and able
to read at 340 ┬▒ 20 nm.

SAMPLES

Plasma collected by standard procedures. Use sodium citrate as anticoagulant.
Plasma fibrinogen is stable for 2 days at 2-8┬░C.

Biosystems Coagulation reagents include the main diagnostic tests and they are characterized by high stability and a good performance using low volume consumption.

These reagents can be performed in semiautomatic and automatic coagulation instruments, independently of the kind of clot detection method (optical and mechanic).

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