Biosystems Transferrin Reagent

Biosystems Transferrin Reagent

A reagent blank should be done every day and a calibration at least every 2 months, after reagent lot change or as required by quality control procedures.Serum or plasma transferrin is stable for 7 days at 2-8C.

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Biosystems Transferrin Reagent

Reagents for the measurement of transferrin concentration in human serum or plasma.

Theobtained values are useful as an aid in the diagnosis and treatment of diseases such as iron deficiency anemia and hemochromatosis..

This reagent is for use in the BioSystems BA analyzers or in other analyzer with similar performance characteristics.

CLINICAL SIGNIFICANCE

Iron is normally transported via the specific binding of Fe3+ by transferrin in blood plasma. The specific iron uptake is regulated according to the individual needs of the various cells.

Elevated transferrin values are found in the presence of iron deficiency (particularly in pregnancy).

The transferrin level may also be raised by drug-based induction

Low transferrin values are found in infectious diseases, malignant tumors, nephrotic syndrome and cirrhosis3,5

Clinical diagnosis should not be made on the findings of a single test result, but should integrate both clinical and laboratory data.

Biosystems Transferrin Reagent

PRINCIPLE OF THE METHOD

Transferrin in the sample precipitates in the presence of anti-human transferrin antibodies.

The light scattering of the antigen-antibody complexes is proportional to the transferrin concentration and can be measured by turbidimetry1,2

STORAGE AND STABILITY

Store at 2-8 ºC.
Components are stable once opened until the expiry date marked in the label if they are stored well closed and care is taken to prevent contamination during their use.

İn board stability: Reagents open and kept in the refrigerated compartment of the analyzer are stable 2 months.

Indications of deterioration: Absorbance of the blank over the limit indicated in “Test Parameters”.

Biosystems Transferrin Reagent

ADDITIONAL MATERIALS REQUIRED (NOT PROVIDED)

Protein Calibrators (BioSystems cod. 31075). The set contains 5 different levels and it should be used to prepare the calibration curve. The calibrators are supplied ready to use.

REAGENT PREPARATION

Reagents are provided ready to use.

SAMPLES

Serum or plasma collected by standard procedures. Use heparin or EDTA as anticoagulants.

Lipemic samples are not suitable for testing.

Serum or plasma transferrin is stable for 7 days at 2-8C.

CALIBRATION

A reagent blank should be done every day and a calibration at least every 2 months, after reagent lot change or as required by quality control procedures.

Biosystems Transferrin Reagent

It is recommended to use the Protein Control Serum level I (cod. 31211) and II (cod. 31212) to verify the accuracy of the measurement procedure.

Each laboratory should establish its own internal Quality Control scheme and procedures for corrective action if control results are not within the acceptable limits.

REFERENCE VALUES

Serum and plasma, adults3
: 200 – 360 mg/dL = 25.2 – 45.4 mol/L.
This range is given for orientation only; each laboratory should establish its own reference range.

Biosystems Transferrin Reagent

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