Biosystems Cholesterol Reagent
A. Reagent. Pipes 35 mmol/L, sodium cholate 0.5 mmol/L, phenol 28 mmol/L, cholesterol
esterase 0.2 U/mL, cholesterol oxidase 0.1 U/mL, peroxidase 0.8 U/mL,
4-aminoantipyrine 0.5 mmol/L, pH 7.0.
S. Cholesterol Standard. Cholesterol 200 mg/dL (5,18 mmol/L). Aqueous primary standard.
Store at 2-8ºC.
Reagent and Standard are stable until the expiry date shown on the label when stored tightly
closed and if contaminations are prevented during their use.
Indications of deterioration:
Reagent: Presence of particulate material, turbidity, absorbance of the blank over 0.200 at
500 nm (1 cm cuvette).
Standard: Presence of particulate material, turbidity.
Reagent and Standard are provided ready to use.
Thermostatic water bath at 37ºC
Analyzer, spectrophotometer or photometer able to read at 500 20 nm
Serum or plasma collected by standard procedures.
Cholesterol is stable for 7 days at 2-8ºC. Heparin, EDTA, oxalate and fluoride may be used as
Cholesterol is a steroid of high molecular weight and possesses the cyclopentanophenanthrene
skeleton. Dietary cholesterol is partially absorbed and it is also synthesized by the liver and
other tissues. Cholesterol is transported in plasma by lipoproteins. It is excreted unchanged into
bile or after transformation to bile acids.
Increased total cholesterol values are associated with a progressively escalating risk of
atherosclerosis and coronary artery disease5,6
Clinical diagnosis should not be made on the findings of a single test result, but should integrate
both clinical and laboratory data.